A comparative study on the extraction effects of common agents on collagen-based binders in mural paintings

Abstract In this paper, a comparative study was conducted on the extraction effects of six agents for collagen-based mural painting binders. These were used to extract residual proteins in non-aged and thermal aged samples. The protein efficiencies different extracting quantitatively determined by bicinchoninic acid (BCA) method, then processed multivariate analysis variance (MANOVA). impact process structure characterized sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), ultraviolet absorption spectrum (UV) circular dichroism (CD). results showed that, both samples, efficiency 2 M guanidine hydrochloride (GuHCl) significantly higher than other five agents, with less damage during process.